Increased NO bioavailability in aging male rats by genistein and exercise training: using 4, 5-diaminofluorescein diacetate

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Increased NO bioavailability in aging male rats by genistein and exercise training: using 4, 5-diaminofluorescein diacetate

BACKGROUND Several kinds of anti-oxidants have drawn a lot of intention for their benefits on vascular protection. In addition, it has been demonstrated that exercise training could improve endothelial function by up-regulating endothelial nitric oxide synthase (eNOS) protein. Therefore, the present study aims to investigate the effects of genistein, a potent phyto-antioxidant, and exercise tra...

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Exercise Training suppresses vascular fibrosis in aging obesity induced rats

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Brain SERT Expression of Male Rats Is Reduced by Aging and Increased by Testosterone Restitution

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Improved measurements of intracellular nitric oxide in intact microvessels using 4,5-diaminofluorescein diacetate.

4,5-Diaminofluorescein diacetate (DAF-2 DA) has been widely used for the measurement of nitric oxide (NO) in living cells and tissues. We previously established a method that demonstrated platelet activating factor (PAF)-induced endothelial NO production in intact venules using DAF-2 DA. In previous applications, the loading dye was removed from the extracellular space before NO measurements. H...

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ژورنال

عنوان ژورنال: Reproductive Biology and Endocrinology

سال: 2009

ISSN: 1477-7827

DOI: 10.1186/1477-7827-7-93